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Biologie du développement
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Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad
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Biologie du développement
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Journal JoVE Biologie du développement
Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

Dissection and Live-Imaging of the Late Embryonic Drosophila Gonad

DOI:
10.3791/61872-v

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09:08 min

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October 17, 2020

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, , ,
1Cell and Developmental Biology Department and the Penn Institute for Regenerative Medicine, Perelman School of Medicine,University of Pennsylvania

Chapitres

  • 00:05Introduction
  • 00:46Day-of-Dissection Preparation
  • 02:35Dissection
  • 04:53Imaging
  • 06:46Results: The Live-Imaging Results of the Cultured Gonads, Their Health, and Niche Compaction
  • 08:15Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we provide a dissection protocol required to live-image the late embryonic Drosophila male gonad. This protocol will permit observation of dynamic cellular processes under normal conditions or after transgenic or pharmacological manipulation.

Tags

Keywords: Live ImagingGonadogenesisDissectionDrosophila EmbryoVitelline MembraneRinger’s SolutionHeptane GlueTungsten NeedlePoly-D-LysineCover Slip

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