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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
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Biologie du développement
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Journal JoVE Biologie du développement
Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

DOI:
10.3791/61885-v

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06:08 min

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October 20, 2020

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, , ,
1Department of Applied Biosciences, Graduate School of Agriculture,Kyoto University

Chapitres

  • 00:04Introduction
  • 00:41Egg Collection and Microinjection
  • 03:04Mating
  • 03:42Genotyping
  • 04:31Results: Dry Injection of T. domestica Eggs
  • 05:37Conclusion

Summary

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Traduction automatique

We provide a detailed protocol for rearing, microinjection of eggs and for efficient mating of the firebrat Thermobia domestica to generate and maintain mutant strains after genome editing.

Tags

Thermobia DomesticaFirebratEgg MicroinjectionGenome EditingInsect ModelCulture MaintenanceRNAiTransgenesisLaboratory Model

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