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Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

DOI:
10.3791/61894-v

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14:04 min

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January 16, 2021

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, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Chapitres

  • 00:00Introduction
  • 01:51Preparation of 13C Stock
  • 02:24Addition of I3C to Protein Stock
  • 03:18Making a Rounded Probe
  • 04:04Making a Seed Stock
  • 06:33Setting Up an rMMS Screen
  • 08:08Data Processing
  • 11:21Representative Results
  • 13:22Conclusion

Summary

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This article presents a method to generate protein crystals derivatized with I3C (5-amino-2,4,6-triiodoisophthalic acid) using microseeding to generate new crystallization conditions in sparse matrix screens. The trays can be set up using liquid dispensing robots or by hand.

Tags

Protein CrystalDerivatizationI3CRandom Microseed Matrix ScreeningX-ray CrystallographyPhasing MoleculeAnomalous SignalSingle Wavelength Anomalous Discussion PhasingLithium I3CProtein CrystallizationStructure Solution

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