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Immunologie et infection
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Fecal (micro) RNA Isolation
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Immunologie et infection
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Journal JoVE Immunologie et infection
Fecal (micro) RNA Isolation

Fecal (micro) RNA Isolation

DOI:
10.3791/61908-v

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05:35 min

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October 28, 2020

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, ,
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Chapitres

  • 00:05Introduction
  • 00:39Feces Resuspension and Organic Extraction
  • 01:52Final RNA Isolation
  • 03:45Elution of the RNA with Nuclease-Free Water
  • 04:09Results: Electrophoresis Assay and Nanodrop Analysis of Extracted RNA
  • 05:00Conclusion

Summary

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This protocol isolates high quality total RNA from fecal samples of animal and human subjects. A commercial miRNA isolation kit is used with significant adaption to isolate pure RNA with optimized quantity and quality. The RNA isolates are good for most downstream RNA assays such as sequencing, micro-array, and RT-PCR.

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FecalMicroRNARNA IsolationDPBSAcid Phenol ChloroformEthanolFilter CartridgeWash Solution

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