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Sciences du comportement
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An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae
Journal JoVE
Sciences du comportement
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Journal JoVE Sciences du comportement
An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae

An Improved Assay and Tools for Measuring Mechanical Nociception in Drosophila Larvae

DOI:
10.3791/61911-v

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07:17 min

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October 29, 2020

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1Department of Genetics,The University of Texas MD Anderson Cancer Center, 2Neuroscience Graduate Program, Graduate School of Biomedical Sciences,The University of Texas MD Anderson Cancer Center, 3Genetics and Epigenetics Graduate Program, Graduate School of Biomedical Sciences,The University of Texas MD Anderson Cancer Center

Chapitres

  • 00:04Introduction
  • 00:56Mechanical Probe Construction
  • 02:26Preparation of Larvae
  • 03:16Mechanical Nociception Assay
  • 04:33Results: Representative Mechanical Nociception and Hypersensitivity
  • 06:33Conclusion

Summary

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The goal of this protocol is to show how to perform an improved assay for mechanical nociception in Drosophila larvae. We use the assay here to demonstrate that mechanical hypersensitivity (allodynia and hyperalgesia) exists in Drosophila larvae.

Tags

Keywords: Mechanical NociceptionDrosophila LarvaeCustom ToolsNitinol FilamentsDose-response CurvesProbe CalibrationForce MeasurementPressure ConversionLarval SortingThird Instar Stage

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