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Diferenciação de células precursoras neurais induzidas por campo elétrico em dispositivos microfluidos
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices
DOI:

07:15 min

April 14, 2021

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Chapitres

  • 00:05Introduction
  • 00:46Design and Fabrication of the Multichannel, Optically Transparent, Electrotactic Chip and Coating Poly-L-lysine on the Substrate in the Cell Culture Regions
  • 02:49Coating Poly-L-lysine on the Substrate in the Cell Culture Regions and Preparation of the Salt Bridge Network
  • 04:47Setup of the Microfluidic System for DC Pulse Stimulation
  • 05:55Results: Differentiation of the mNPC Cells in the Control Group and in the DC Pulse Stimulation Group at DIV 3, 7, and 14
  • 06:43Conclusion

Summary

Traduction automatique

Neste estudo, apresentamos um protocolo para a diferenciação de células neurais e progenitoras (NPCs) induzidas unicamente pela estimulação de pulso de corrente direta (DC) em um sistema microfluido.

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