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In vivo Evaluering af mucociliary clearance hos mus
Journal JoVE
Biologie
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Journal JoVE Biologie
In vivo Evaluation of Mucociliary Clearance in Mice

In vivo Evaluering af mucociliary clearance hos mus

DOI:
10.3791/61929-v

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06:35 min

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December 18, 2020

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,
1Department of Developmental Biology,University of Pittsburgh School of Medicine

Chapitres

  • 00:04Introduction
  • 00:39Mouse Intubation and Instillation
  • 02:06Single Photon Emission Computed Tomography and Computed Tomography (SPECT/CT) Imaging
  • 03:10Analysis
  • 04:48Results: Representative Mouse Mucociliary Clearance (MCC) Evaluation
  • 05:56Conclusion

Summary

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Traduction automatique

I denne publikation beskriver vi protokoller til vurdering af luftvejes mucociliary clearance (MCC) hos mus in vivo ved hjælp af dual-modality radionuclide imaging. Denne protokol er designet til en enkelt foton emission computertomografi (SPECT) og computertomografi (CT) erhvervelse protokol ved hjælp af musen hele kroppen (MWB) collimators i en dobbelt SPECT / CT-system.

Tags

Mucociliary ClearanceIn Vivo EvaluationMiceRadionuclidesSPECT/CT ImagingAnesthesiaIntubationTechnetium Sulfur Colloid3D ReconstructionImage Analysis

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