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Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic
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1Pneumology laboratory, I3 Group, GIGA Research Center,University of Liège, 2Department of ENT,University Hospital of Liège, 3Department for Occupational Safety and Health, Biosafety and Biosecurity Unit,University of Liège, 4Division of Respirology, Department of Pediatrics,University of British Columbia and British Columbia Children’s Hospital, 5Division of Cardiology, Department of Pediatrics,University Hospital of Liège, 6Department of Pneumology,University Hospital of Liège, 7Division of Respirology, Department of Pediatrics,University Hospital of Liège
Chapitres
- 00:05Introduction
- 01:41Respiratory Ciliated Epithelium Sample Collection
- 02:35Respiratory Ciliated Epithelium Processing and Preparation
- 03:55Respiratory Ciliated Edge Selection and Recording
- 05:18Ciliated Edge Analysis
- 06:59Results: Representative Nasal Brush Processing Analysis
- 08:34Conclusion
To guarantee a successful and high-quality ciliary functional analysis for PCD diagnosis, a precise and careful method for respiratory epithelium sampling and processing is essential. To continue providing PCD diagnostic service during the COVID-19 pandemic, the ciliary videomicroscopy protocol has been updated to include appropriate infection control measures.
