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Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

DOI:
10.3791/61976-v

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08:44 min

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February 21, 2021

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, ,
1Center for Advanced Research in Sleep Medicine,Recherche CIUSSS-NIM, 2GELIFES,University of Groningen, 3Department of Neuroscience,Université de Montréal

Chapitres

  • 00:05Introduction
  • 00:42Intracortical Adeno-Associated Virus (AAV) Injection
  • 04:04Electrocorticographic (ECoG) and Electromyographic (EMG) Electrode Implantation and Recording
  • 07:28Results: Representative AAV Cofilin Targeting within the Mouse Cerebral Cortex
  • 08:08Conclusion

Summary

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This article describes a protocol for the manipulation of molecular targets in the cerebral cortex using adeno-associated viruses and for monitoring the effects of this manipulation during wakefulness and sleep using electrocorticographic recordings.

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ElectrocorticographyAdeno-associated VirusCofilinMiceStereotaxic SurgeryBrain Region TargetingVirus Injection

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