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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Biologie
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Journal JoVE Biologie
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

DOI:
10.3791/62008-v

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09:08 min

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December 22, 2020

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, , , , , , , , , ,
1The Graduate Center – Advanced Science Research Center, Neuroscience Initiative,The City University of New York, 2The City College of New York, CUNY, 3The Bronx High School of Science, 4The Graduate Center – Advanced Science Research Center, Structural Biology Initiative,The City University of New York, 5The Graduate Center – Advanced Science Research Center, MALDI MS Imaging Joint Core Facility,The City University of New York

Chapitres

  • 00:04Introduction
  • 00:46Tissue Preparation
  • 01:18Cryosection Acquisition
  • 03:24Matrix Preparation
  • 04:13Matrix Deposition
  • 06:16Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
  • 07:37Conclusion

Summary

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The goal of this protocol is to provide detailed guidance on the sample preparation when planning for experiments using MALDI MSI to maximize metabolic and molecular detection in biological samples.

Tags

MALDI Imaging Mass SpectrometryMetabolite Abundance And DistributionCryosectioningSlide PreparationMALDI Slide ConductivitySample Drying

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