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Characterization of a Novel Human Organotypic Retinal Culture Technique
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Journal JoVE Médecine
Characterization of a Novel Human Organotypic Retinal Culture Technique

Characterization of a Novel Human Organotypic Retinal Culture Technique

DOI:
10.3791/62046-v

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05:51 min

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June 09, 2021

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, , ,
1Buchanan Ocular Therapeutics Unit, Department of Ophthalmology and the New Zealand National Eye Centre,University of Auckland

Chapitres

  • 00:05Introduction
  • 00:53Media Preparation and Extraction of the Sandwich Retinal Explants from the Eyecup
  • 03:02Results: Observation of Cellular Integrity of Retinal Explants Using H&E, IHC and Analysis of Cytokine Release Using Magnetic Assay
  • 04:53Conclusion

Summary

Traduction automatique

Traduction automatique

This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera.

Tags

Organotypic Retinal CultureRetinal ExplantRetinal IntegrityRetinal DiseasesDrug TestingDulbecco’s Modified Eagle MediumAntibioticsAntimycoticsDissectionSample CollectionScleraOptic Nerve Head

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