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Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
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Médecine
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Journal JoVE Médecine
Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

DOI:
10.3791/62058-v

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05:16 min

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December 22, 2020

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, , , , , , , ,
1University Hospital Munich, Department of Medicine I,Ludwig Maximilians University (LMU) Munich, 2Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine,University Hospital Munich, Ludwig Maximilians University (LMU) Munich, 3German Center for Cardiovascular Research (DZHK), Partner Site Munich,Munich Heart Alliance

Chapitres

  • 00:04Introduction
  • 00:51Organ Harvest and Tissue Preparation
  • 02:23Whole-Mount Immunofluorescence Staining
  • 03:13Confocal Microscopy
  • 04:19Results: Reconstruction of SAN and AVN
  • 04:49Conclusion

Summary

Traduction automatique

Traduction automatique

We provide a step-by-step protocol for whole-mount immunofluorescence staining of the sinoatrial node (SAN) and atrioventricular node (AVN) in murine hearts.

Tags

Keywords: Whole-mount ImmunofluorescenceConfocal Imaging3D ReconstructionSinoatrial NodeAtrioventricular NodeMouseMicro-dissectionPBSParaformaldehydeSucroseTriton X-100Connexin 43HTN4Secondary AntibodiesDAPIConfocal Microscopy

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