/

/

小鼠窦房和房室结的全卡口免疫荧光染色、共聚焦成像和 3D 重建

Journal JoVE
Médecine

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Médecine

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

Please note that all translations are automatically generated. Click here for the English version.

小鼠窦房和房室结的全卡口免疫荧光染色、共聚焦成像和 3D 重建

DOI:

10.3791/62058-v

•

05:16 min

•

December 22, 2020

•

Ruibing Xia^2,3, Julia Vlcek², Julia Bauer^2,3, Stefan Kääb³, Hellen Ishikawa-Ankerhold², Dominic Adam van den Heuvel², Christian Schulz^2,3, Steffen Massberg^2,3, Sebastian Clauss^2,3

¹University Hospital Munich, Department of Medicine I,Ludwig Maximilians University (LMU) Munich, ²Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine,University Hospital Munich, Ludwig Maximilians University (LMU) Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich,Munich Heart Alliance

Chapitres

00:04Introduction
00:51Organ Harvest and Tissue Preparation
02:23Whole-Mount Immunofluorescence Staining
03:13Confocal Microscopy
04:19Results: Reconstruction of SAN and AVN
04:49Conclusion

Summary

Traduction automatique

我们为鼠心脏窦房结（SAN）和房室结（AVN）的全安装免疫荧光染色提供了分步方案。

Tags

Whole-mount Immunofluorescence Confocal Imaging 3D Reconstruction Sinoatrial Node Atrioventricular Node Mouse Micro-dissection PBS Paraformaldehyde Sucrose Triton X-100 Connexin 43 HTN4 Secondary Antibodies DAPI Confocal Microscopy

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

在的鼠标Aortocaval瘘的技术方面

共聚焦成像在肝癌细胞中观察到的异常有丝分裂细胞死亡相关

小鼠膈肌功能诊断超声成像

玫瑰红Photothrombosis共聚焦光学成像<em>在体内</em>：单支脑卒中模型

单元位置的Imaging-和流式细胞仪为基础的分析和3D球体黑色素瘤细胞周期

可读机辅助重建术后评估指南

腹部小鼠心脏移植模型中的血液回路重建

在健康和受伤的老鼠心脏中为三维纤维细胞组织进行精炼的清晰组织清除

小鼠窦房和房室结常驻心脏巨噬细胞的分离和培养

微电极阵列记录中天节点发射率，以识别小鼠内在心脏起搏缺陷

Read Article