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Stereocilia Bundle Imaging med Nanoskala Opløsning i Live Mammalian auditive hårceller
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Neurosciences
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Journal JoVE Neurosciences
Stereocilia Bundle Imaging with Nanoscale Resolution in Live Mammalian Auditory Hair Cells

Stereocilia Bundle Imaging med Nanoskala Opløsning i Live Mammalian auditive hårceller

DOI:
10.3791/62104-v

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06:47 min

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January 21, 2021

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, ,
1Department of Physiology, College of Medicine,University of Kentucky, 2Universidad Nacional de Colombia

Chapitres

  • 00:05Introduction
  • 00:42Nanopipette Testing
  • 01:36Testing Imaging Resolution with Calibration Standards for Atomic Force Microscopy (AFM)
  • 03:29Auditory Hair Cell Imaging
  • 04:51Results: Representative HPICM Stereocilia Bundle Imaging
  • 06:11Conclusion

Summary

Traduction automatique

Traduction automatique

Her præsenterer vi en protokol til Hopping Probe Ion Conductance Microscopy (HPICM), en ikke-kontakt scanning sonde teknik, der tillader nanoskala billeddannelse af stereocilia bundter i levende auditive hårceller.

Tags

Keywords: StereociliaHair CellsNanoscale ResolutionLive Cell ImagingHPICMNanopipettesCalibration StandardZ ApproachSurface Topography

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