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Bioengineering

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JoVE Journal Bioengineering
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
 

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Article DOI: 10.3791/62148-v • 09:36 min • February 3rd, 2021
February 3rd, 2021 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Bioengineering, Rice University
* These authors contributed equally

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Detailed instructions are provided to build an open-source, modular fluorimeter that is compatible with many low-cost heaters to perform real-time, quantitative isothermal nucleic acid amplification.

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Keyword Extraction: Open-source Miniature Fluorimeter Isothermal Nucleic Acid Amplification Resource-limited Settings Molecular Diagnostics Isothermal Amplification Optics Enclosure Test Board Focal Length Lens Long Pass Filter Emission Filter Dichroic Mirror Beam Splitter Excitation Filter LED
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