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JoVE Journal Developmental Biology
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
 

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Article DOI: 10.3791/62163-v • 06:56 min • January 21st, 2021
January 21st, 2021 •
1, 1, 1, 1
1Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia

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We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.

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Keywords: Cell Dissociation Tongue Epithelium Tongue Mesenchyme Connective Tissue Embryonic Day 12.5 8-week-old Mice Single Cell RNA Sequencing Primary Stem Cell Cultures Tissue Separation Enzymatic Digestion Tyrode's Solution Dispase Collagenase
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