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Chapitres
- 00:04Introduction
- 00:46Primary Retinal Ganglion Cell Culture Derived from Zebrafish Embryos
- 04:45In Vitro ROS Imaging of Cultured RGC Neurons
- 06:13In Vitro ROS Imaging of Developing Embryos
- 07:40Results: H2O2 Imaging in Cultured and in Vivo Zebrafish RGCs with roGFP2-Orp1
- 08:53Conclusion
This protocol describes the use of a genetically encoded hydrogen peroxide (H2O2)-biosensor in cultured zebrafish neurons and larvae for assessing the physiological signaling roles of H2O2 during nervous system development. It can be applied to different cell types and modified with experimental treatments to study reactive oxygen species (ROS) in general development.
