Journal
/
Médecine
/
Functional Characterization of Endogenously Expressed Human RYR1 Variants
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Functional Characterization of Endogenously Expressed Human RYR1 Variants

Functional Characterization of Endogenously Expressed Human RYR1 Variants

DOI:
10.3791/62196-v

•

07:59 min

•

June 09, 2021

•

, ,
1Department of Biomedicine,Basel University Hospital, 2Department of Life Sciences,University of Ferrara, 3Department of Anesthesia,Basel University Hospital

Chapitres

  • 00:04Introduction
  • 01:00Intracellular Cell Culture Ca2+ Measurement
  • 02:30Intracellular Single Cell Ca2+ Measurement
  • 03:56Human Myotube Preparation
  • 04:40Human Myotube Inducible Calcium ([Ca2+]i) Measurement
  • 05:33Results: Representative Single Cell Ca2+ Release in EBV-Immortalized Human B Cells and Isolated Human Myotubes
  • 07:11Conclusion

Summary

Traduction automatique

Traduction automatique

Here methods used to study the functional effect of RYR1 mutations endogenously expressed in Epstein Barr Virus immortalized human B-lymphocytes and muscle biopsy derived satellite cells differentiated into myotubes are described.

Tags

Keywords: RYR1 VariantsCalcium HomeostasisEBV-transformed B LymphocytesFura-2 AMKrebs Ringer’s SolutionCalcium Release ResponseSingle Cell AnalysisSpectrofluorometerPerfusion ChamberFluorescent Microscopy

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/62196?article_type=v

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson’s Disease
check_url/fr/62196?article_type=v

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
check_url/fr/62196?article_type=v

Network Analysis of the Default Mode Network Using Functional Connectivity MRI in Temporal Lobe Epilepsy
check_url/fr/62196?article_type=v

In vitro Functional Characterization of Mouse Colorectal Afferent Endings
check_url/fr/62196?article_type=v

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion
check_url/fr/62196?article_type=v

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
check_url/fr/62196?article_type=v

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
check_url/fr/62196?article_type=v

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
check_url/fr/62196?article_type=v

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
check_url/fr/62196?article_type=v

A Fluorescence-based Assay for Characterization and Quantification of Lipid Droplet Formation in Human Intestinal Organoids

Read Article