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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
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Médecine
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Journal JoVE Médecine
Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

DOI:
10.3791/62236-v

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08:43 min

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May 07, 2021

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, , , , , ,
1University Hospital Munich, Department of Medicine I,Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich,Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

Chapitres

  • 00:05Introduction
  • 00:58Microdissection of SAN and AVN
  • 03:14Digestion
  • 04:02Magnetic Enrichment of CD45 and Sample Staining
  • 05:28Cell Sorting and Gating Strategy
  • 06:10Results: Identification of SAN and AVN and Analysis of Sorted Cardiac Macrophages
  • 08:17Conclusion

Summary

Traduction automatique

Traduction automatique

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

Tags

Cardiac MacrophagesSinus NodeAtrioventricular NodeMicrodissectionFluorescence-activated Cell SortingCardiac ElectrophysiologyMouse

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