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颗粒模板化乳化可实现无微流体液滴检测

Journal JoVE
Biologie

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Journal JoVE Biologie

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

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颗粒模板化乳化可实现无微流体液滴检测

DOI:

10.3791/62248-v

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11:03 min

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March 09, 2021

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Daniel W. Weisgerber, Makiko N. Hatori, Adam R. Abate²

¹Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, ²Chan Zuckerberg Biohub

Chapitres

00:04Introduction
00:44Preparation of Hydrogel Particles for Particle Templated Emulsification
02:31Fabrication of the Custom Microfluidic Device
04:37Fabrication of Templating Particles
06:26Particle Templated Emulsification
08:26Digital Droplet PCR and Analysis
09:02Results: Sample Encapsulation Using Particle Templated Emulsification (PTE)
10:30Conclusion

Summary

Traduction automatique

油包水液滴测定可用于分析化学、酶进化和单细胞分析，但通常需要微流体才能形成液滴。在这里，我们描述了颗粒模板化乳化，这是一种进行液滴测定的无微流体方法。

Tags

Keywords: Particle Templated Emulsification Microfluidic-free Droplet Assays Digital Droplet PCR Emulsification Polyacrylamide Particles Photoresist PDMS Microfluidic Fabrication Photomask Post Exposure Baking PGMEA Isopropanol Plasma Treatment

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