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Cryo-EM and Single-Particle Analysis with Scipion
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

DOI:
10.3791/62261-v

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09:06 min

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May 29, 2021

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, , , , , , , , , , , , , , , , , , ,
1Centro Nacional de Biotecnología,Campus Universidad Autónoma de Madrid, 2Faculty of Informatics,Masaryk University, 3Institute of Computer Science,Masaryk University, 4Campus Urbanización Montepríncipe,Universidad San Pablo CEU

Chapitres

  • 00:05Introduction
  • 00:53Project Creation and Data Import
  • 01:42Movie Alignment: From Movies to Micrographs
  • 02:37Particle Picking
  • 05:28Evaluation and Post-Processing
  • 06:53Results: Representative Single-Particle Analysis
  • 08:26Conclusion

Summary

Traduction automatique

Traduction automatique

Single-particle analysis in cryo-electron microscopy is one of the main techniques used to determine the structure of biological ensembles at high resolution. Scipion provides the tools to create the whole pipeline to process the information acquired by the microscope and achieve a 3D reconstruction of the biological specimen.

Tags

Cryo-EMSingle-particle AnalysisScipionImage ProcessingWorkflowInteroperabilityTraceabilityReproducibilityCryo-electron TomographyAtomic ModelingMovie AlignmentOptical FlowParticle PickingMicrographPower Spectral DensityTrajectory

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