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注射重组狐尾花叶病毒和甘蔗花叶病毒传染性克隆直接农种玉米幼苗
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
DOI:

05:56 min

February 27, 2021

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Chapitres

  • 00:04Introduction
  • 00:39Preparation of Seedling and Agrobacterium
  • 02:06Injection of Agrobacterium and Infection Detection
  • 03:36Results: Detection of Infection Lesion and Gene Suppression Analysis
  • 05:22Conclusion

Summary

Traduction automatique

提出了一种基于 农杆菌的注射(农用注射)方案,用于将狐尾花叶病毒和甘蔗花叶病毒克隆接种到玉米幼苗中。以这种方式接种会导致病毒感染、病毒诱导的标记基因沉默以及 GFP 的病毒过表达。

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