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In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow
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Chapitres
- 00:04Introduction
- 00:44Design and Fabrication of a 3D Bifurcation of the Human Carotid Artery Model
- 02:18Human Umbilical Vein Endothelial Cell (HUVEC) Seeding
- 03:26Closed-Circuit Configuration: Perfusion Experiment and Imaging
- 04:42Open-Circuit Configuration: Model Washing, Data Acquisition, and Analysis
- 05:36Results: Microscopic Analysis of 3D Carotid Artery Model-Seeded Endothelial Cells
- 06:17Conclusion
Here, we present a new protocol to study and map the targeted deposition of drug carriers to endothelial cells in fabricated, real-sized, three-dimensional human artery models under physiological flow. The presented method may serve as a new platform for targeting drug carriers within the vascular system.
