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1Department of Physiology & The Institute for Digital Medicine (WisDM),National University of Singapore, 2College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute,National University of Singapore, 4Institute of Bioengineering and Nanotechnology,A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP,Singapore-MIT Alliance for Research and Technology
Chapitres
- 00:04Introduction
- 00:45Portal Vein Cannulation
- 03:54Liver Perfusion, Digestion, Hepatocyte Isolation
- 05:30Results: Estimation of Purity and Function of Isolated Hepatocytes
- 07:07Conclusion
Denne protokol beskriver brugen af et specielt intravenøst kateter, standardiseret steril engangsslange, temperaturregulering suppleret med realtidsovervågning og et alarmsystem til to-trins kollagenaseperfusionsprocedure for at forbedre konsistensen i levedygtigheden, udbyttet og funktionaliteten af isolerede primære rotte hepatocytter.
