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用于SARS-CoV-2检测和定量的两步逆转录液滴数字PCR方案
 
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用于SARS-CoV-2检测和定量的两步逆转录液滴数字PCR方案

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

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这项工作总结了使用双色ddPCR系统开发SARS-CoV-2检测的不同检测方法的步骤。这些步骤很详细，并且已经包括有关如何改进测定和实验性能的说明。这些检测可用于多种SARS-CoV-2 RT-ddPCR应用。

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本月在JoVE上，第169期，单工，双工，三链体探针混合物，四链体，测定，SARS-CoV-2，RT-ddPCR，检测，多重
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