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Immunologie et infection
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Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis
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Immunologie et infection
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Journal JoVE Immunologie et infection
Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

Isolating Brown Adipocytes from Murine Interscapular Brown Adipose Tissue for Gene and Protein Expression Analysis

DOI:
10.3791/62332-v

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07:07 min

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March 12, 2021

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, ,
1Masonic Medical Research Institute

Chapitres

  • 00:04Introduction
  • 01:09Brown Adipocytes (BAs) Isolation Procedure
  • 02:14RNA and Protein Isolation from BAs
  • 04:28Results: Validation of BA Isolation Method with Microscopy and Gene Expression Analysis
  • 06:15Conclusion

Summary

Traduction automatique

Traduction automatique

This study describes a new method of isolating murine brown adipocytes for gene and protein expression analysis.

Tags

Keywords: Brown AdipocytesGene ExpressionProtein ExpressionAdipocyte IsolationIodixanol GradientTRIzolRNA IsolationProtein Isolation

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