/

/

神经节苷脂提取、纯化和分析

Journal JoVE
Biochimie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biochimie

Ganglioside Extraction, Purification and Profiling

Please note that all translations are automatically generated. Click here for the English version.

神经节苷脂提取、纯化和分析

DOI:

10.3791/62385-v

•

10:05 min

•

March 12, 2021

•

Mitchell J. Porter*¹, Gao-Lan Zhang*¹, Ronald L. Schnaar²

¹Department of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, ²Department of Neuroscience,Johns Hopkins University School of Medicine

Chapitres

00:04Introduction
01:36Small Scale Ganglioside Extraction and Partial Purification
03:35Large Scale Ganglioside Extraction and Purification
05:52Ganglioside Resolution and Staining
08:02Results: Small and Large Scale Ganglioside Extraction and Partial Purification
09:18Conclusion

Summary

Traduction automatique

神经节苷脂是含唾液酸的鞘糖脂，在大脑中特别丰富。它们的两性性质需要有机/水提取和纯化技术，以确保最佳的回收率和准确的分析。本文概述了分析和制备规模的神经节苷脂提取，纯化和薄层色谱分析。

Tags

Keywords: Ganglioside Extraction Ganglioside Purification Ganglioside Profiling Cell Surface Lipids Nerve Cell Lipids Cell Signaling Cell-cell Recognition Polar Lipid Analysis Reverse-phase Cartridge Chromatography Solvent Extraction Partitioning Solid-phase Extraction

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

实验室规模的生产和治疗性抗体的纯化

S100A12的表达，纯化和抗菌活性

在利什曼原虫、人体细胞和小鼠睾丸中的 Polysome 分析

光谱分析 TRPV1 的纯化与重构

哺乳动物 Bestrophin 离子通道的表达与纯化

台湾绿蜂胶的提取与分析

真核硼酸背液转运体的表达、增溶及纯化

血管紧张蛋白II图解的硝酸分析与鉴定

DNA序列识别利用高通量原酶分析的DNAPrimase

通过低温BN-MS分析进行高分辨率复杂分析

Read Article