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Hurtig antimikrobiel følsomhedstest ved stimuleret Raman-spredningsbilleddannelse af deuteriuminkorporering i en enkelt bakterie
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Journal JoVE Biologie
Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Hurtig antimikrobiel følsomhedstest ved stimuleret Raman-spredningsbilleddannelse af deuteriuminkorporering i en enkelt bakterie

DOI:
10.3791/62398-v

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12:08 min

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February 14, 2022

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, ,
1Department of Electrical and Computer Engineering,Boston University, 2Boston University Photonics Center,Boston University, 3Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 4Department of Biomedical Engineering,Boston University, 5Department of Chemistry,Boston University

Chapitres

  • 00:04Introduction
  • 00:38Deuterium (D2O) Incorporation Treatment in the Presence of Antibiotics
  • 03:50Preparation of Bacteria in Urine Environment and Blood Environment
  • 05:42Stimulated Raman Scattering (SRS) Imaging of D2O Metabolic Incorporation in a Single Bacterium
  • 08:44Results: The Influence of Deuterium Incorporation on Bacterial Growth
  • 11:14Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol præsenterer hurtig antimikrobiel følsomhedstest (AST) assay inden for 2,5 timer ved enkeltcellestimuleret Raman-spredningsbilleddannelse af D2O-metabolisme. Denne metode gælder for bakterier i urin- eller fuldblodsmiljøet, hvilket er transformerende for hurtig enkeltcellet fænotypisk AST i klinikken.

Tags

Keywords: Rapid Antimicrobial Susceptibility TestingStimulated Raman ScatteringDeuterium IncorporationSingle BacteriumBacterial ConcentrationOptical DensityColony Forming UnitsAntibioticMHB MediumSerial DilutionPositive ControlNegative ControlIncubationHomogenization

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