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Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Biochimie
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Journal JoVE Biochimie
Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

DOI:
10.3791/62415-v

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11:52 min

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May 29, 2021

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, , , , , , ,
1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences,University of Leeds, 2Diamond Light Source, Harwell Science and Innovation Campus

Chapitres

  • 00:04Introduction
  • 00:47Performing an Inventory and Screening for Ice and Sample Quality
  • 04:07Single Particle CryoEM Data Collection
  • 09:44Results: Single Particle Cryo-Electron Microscopy: Screening for 3D Classification and Refinement
  • 11:04Conclusion

Summary

Traduction automatique

Traduction automatique

Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.

Tags

Keywords: Single Particle Cryo-electron MicroscopyCryo-EMSample PreparationGrid ScreeningRemote Data CollectionAuto-loaderEPU SoftwareAtlasMembrane ProteinsAmyloid FibrilsNucleic Acid Binding ProteinsViruses

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