Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Introduction
- 00:42Sample Preparation
- 01:17Headspace Solid-phase Microextraction (HS-SPME) Coupled with Gas Chromatography-mass Spectrometry (GC-MS) Data Acquisition
- 02:23Analysis of GC-MS Profile Chromatograms: VOC Identification and Semi-quantification
- 03:20Results: Analysis of HS-SPME/GC-MS Chromatograms, PCA, and Relative VOC Content in Blackcurrant Fruits
- 04:48Conclusion
A headspace solid-phase microextraction-gas-chromatography platform is described here for fast, reliable, and semi-automated volatile identification and quantification in ripe blackcurrant fruits. This technique can be used to increase knowledge about fruit aroma and to select cultivars with enhanced flavor for the purpose of breeding.
Tags
Keywords:
Volatile CompoundsBlackcurrant FruitHeadspace Solid-phase MicroextractionGas Chromatography-mass SpectrometryFruit AromaBreeding ProgramsSemi-automated TechniqueBerry CropsSodium Chloride SolutionCentrifugationInternal StandardGC-MS Auto SamplerHeadspace VialSPME DeviceVolatile DesorptionGC-MS AnalysisRetention TimeMass SpectraKovats Linear Retention IndexPeak AreaInternal StandardBatch Effect Correction
