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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
Journal JoVE
Biologie
Author Produced
This content is Free Access.
Journal JoVE Biologie
Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
DOI:

14:40 min

March 05, 2022

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Chapitres

  • 00:00Introduction
  • 00:07Experimental Workflow
  • 00:38Total RNA Extraction
  • 02:22mRNA Isolation
  • 04:38mRNA Fragmentation
  • 06:09meRIP-Seq
  • 10:21Bisulfite Conversion
  • 12:47Résultats
  • 13:45Conclusion

Summary

Traduction automatique

The role of RNA modifications in viral infections is just starting to be explored and could highlight new viral-host interaction mechanisms. In this work, we provide a pipeline to investigate m6A and m5C RNA modifications in the context of viral infections.

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