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Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements
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Journal JoVE Biologie
Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

DOI:
10.3791/62427-v

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07:19 min

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July 29, 2021

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, , , , , ,
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health, 2Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus,University of Exeter

Chapitres

  • 00:04Introduction
  • 00:31Preparation for Homogenization
  • 01:50Homogenization
  • 05:30Results: Nitrate and Nitrite Levels in Rat Skeletal Muscle Homogenates
  • 06:54Conclusion

Summary

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We present protocols for three different methods for the homogenization of four different muscle groups of rat skeletal muscle tissue to measure and compare the levels of nitrate and nitrite. Furthermore, we compare different sample weights to investigate whether tissue sample size affects the results of homogenization.

Tags

Keywords: Rat Skeletal MuscleNitrateNitriteHomogenizationStop SolutionPotassium FerricyanideN-methylmaleimideSurfactantCentrifugationMethanolBead HomogenizationPulverizer Homogenization

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