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Ved hjælp af fluorescerende Dye, Rhodamine B, at studere parring konkurrenceevne i Mandlige Aedes aegypti Myg
Journal JoVE
Biologie
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Journal JoVE Biologie
Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes

Ved hjælp af fluorescerende Dye, Rhodamine B, at studere parring konkurrenceevne i Mandlige Aedes aegypti Myg

DOI:
10.3791/62432-v

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07:10 min

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May 07, 2021

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, , ,
1Environmental Health Institute,National Environmental Agency

Chapitres

  • 00:04Introduction
  • 00:41Preparation of Male and Female Mosquitoes
  • 01:11Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
  • 01:52Checking for RhB Fluorescence in Male Mosquitoes
  • 02:46Mating Competitiveness Assay
  • 05:36Results: Assessment of Mating Competitiveness in Male Mosquitoes
  • 06:27Conclusion

Summary

Traduction automatique

Traduction automatique

Her præsenterer vi en protokol for undersøgelse parring konkurrenceevne mandlige Aedes aegypti ved hjælp af fluorescerende farvestof som en markør. Kvindelige myg udsættes for både markerede og umærkede mænd til copulation. Post parring, deres spermathecae undersøges under en fluorescens mikroskop til at bestemme deres parring partner.

Tags

Keywords: Rhodamine BAedes AegyptiMating CompetitivenessMosquito Population SuppressionQuality ControlStrain EvaluationMale MosquitoesFemale MosquitoesSucrose SolutionFluorescence MicroscopyMating Assay

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