Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Key Laboratory of Experimental Teratology, Ministry of Education, Institute of Molecular Medicine and Genetics, School of Basic Medical Sciences, Stem Cell and Regenerative Medicine Research Center, Cheeloo College of Medicine,Shandong University, 2The Research Center of Stem Cell and Regenerative Medicine,Shandong University Cheeloo Medical College, School of Basic Medical Sciences, Shandong University
Chapitres
- 00:01Introduction
- 00:47Murine Hepatocyte Isolation by Liver Perfusion and Digestion
- 02:47Hepatocyte Organoids Culture
- 04:29Single Cells from Hepatocyte Organoids
- 07:22siRNA or Plasmid Transfection
- 12:58Representative Results
- 13:58Conclusion
Un sistema di coltura organoide 3D ex vitro a lungo termine è stato stabilito da epatociti di topo. Questi organoidi possono essere passati e manipolati geneticamente dall'infezione da lentivirus di costruzione shRNA/ectopica, trasfezione di siRNA e ingegneria CRISPR-Cas9.
