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Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
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Biologie
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Journal JoVE Biologie
Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

DOI:
10.3791/62447-v

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09:19 min

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March 28, 2021

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, , , , ,
1Department of Environmental Health Sciences,Columbia University Mailman School of Public Health, 2Department of Medicine,Naomi Berrie Diabetes Center, Columbia University

Chapitres

  • 00:04Introduction
  • 00:58Cuvette Preparation
  • 01:53Particle Tracking Instrument Preparation and Nanoparticle Tracking Analysis
  • 04:01NTA Data Processing, Display, and Interpretation
  • 05:29Cuvette Cleaning
  • 06:33Results: Using a Novel NTA Instrument to Estimate EV Size Distribution and Total Particle Concentration
  • 08:33Conclusion

Summary

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We demonstrate how to use a novel nanoparticle tracking analysis instrument to estimate the size distribution and total particle concentration of extracellular vesicles isolated from mouse perigonadal adipose tissue and human plasma.

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Nanoparticle Tracking AnalysisExtracellular VesiclesNTA ParametersSample PreparationCuvetteParticle ConcentrationDilutionFocusFrame RateExposure TimeStir TimeReproducibilityStandardization

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