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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
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Chimie
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Journal JoVE Chimie
A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

DOI:
10.3791/62467-v

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10:30 min

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June 10, 2022

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, ,
1Department of Food and Human Nutritional Sciences,University of Manitoba, 2Richardson Centre for Functional Foods and Nutraceuticals,University of Manitoba

Chapitres

  • 00:09Introduction
  • 00:58Type of Samples
  • 01:09Sample Preparation
  • 03:20Antioxidant Assays
  • 04:31Dilution of Sample Extracts
  • 04:49TEAC Assay
  • 06:51DPPH Assay
  • 07:56Résultats
  • 10:02Conclusion

Summary

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Phenolic acids are important phytochemicals that are present in whole grains. They possess bioactive properties such as antioxidant protective functions. This work aimed at reporting on a generalized method for the HPLC identification, total phenolic content estimation, and determination of the antioxidant capacity of phenolic acids in cereals and legumes.

Tags

Whole GrainsCerealsLegumesPhenolic AcidsAntioxidant CapacityHydroxybenzoic AcidsHydroxycinnamic AcidsFree Soluble Phenolic CompoundsHPLCExtractionCentrifugationFiltration

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