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Etablering af stabile binære kulturer symbiotiske saccharibakterier fra mundhulen
Journal JoVE
Biologie
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Journal JoVE Biologie
Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity

Etablering af stabile binære kulturer symbiotiske saccharibakterier fra mundhulen

DOI:
10.3791/62484-v

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07:32 min

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April 13, 2021

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, ,
1The Forsyth Institute, 2Harvard School of Dental Medicine

Chapitres

  • 00:04Introduction
  • 01:11Preparation Initiating Host Cultures and Sterilizing Equipment
  • 01:55Obtaining Sample of Oral Bacteria
  • 02:30Preparation of Filtrate from Oral Sample
  • 03:28Concentration of the Saccharibacteria Cells by Centrifugation
  • 04:02Infection of Host Cultures with Saccharibacteria-enriched Filtrate
  • 04:47Confirmation of Infection by PCR and Verification of Purity
  • 05:36Results: Confirmation of Infection and Purity of Binary Cultures of Symbiotic Saccharibacteria
  • 06:54Conclusion

Summary

Traduction automatique

Traduction automatique

Vi demonstrerer en metode til at isolere svært at dyrke medlemmer af romanen bakteriel phylum, Saccharibacteria, ved at filtrere plak og co-dyrkning med vært bakterier.

Tags

SaccharibacteriaOral CavityBinary Co-cultureObligate ParasitesHost BacteriaArachnia PropionicaDental PlaqueFiltrationIsolationMicrobiological Techniques

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