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Control of Cell Geometry through Infrared Laser Assisted Micropatterning
Journal JoVE
Bio-ingénierie
Author Produced
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Journal JoVE Bio-ingénierie
Control of Cell Geometry through Infrared Laser Assisted Micropatterning
DOI:

11:04 min

July 10, 2021

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Chapitres

  • 00:00Introduction
  • 01:16Coating Coverslips with PVA
  • 02:09Setting Up Optical Configurations
  • 06:47Mounting the Coverslip and Setting Up the Macro
  • 08:45Résultats
  • 10:15Conclusion

Summary

Traduction automatique

The protocol presented here enables automated fabrication of micropatterns that standardizes cell shape to study cytoskeletal structures within mammalian cells. This user-friendly technique can be set up with commercially available imaging systems and does not require specialized equipment inaccessible to standard cell biology laboratories.

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