Journal
/
Biologie
/
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

Quantification of Cellular Densities and Antigenic Properties using Magnetic Levitation

DOI:
10.3791/62550-v

•

05:25 min

•

May 17, 2021

•

, , , , ,
1Nano Flow Core Facility,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Division of Allergy and Inflammation,Beth Israel Deaconess Medical Center

Chapitres

  • 00:04Introduction
  • 00:43Collection and Preparation of Blood for Positive Rhesus Factor Detection
  • 02:43Cell Analysis Using the Magnetic Levitation Device
  • 03:21Results: Analysis of the Separated Cells
  • 04:57Conclusion

Summary

Traduction automatique

Traduction automatique

This paper describes a magnetic levitation-based method that can specifically detect the presence of antigens, either soluble or membrane-bound, by quantifying changes in the levitation height of capture beads with fixed densities.

Tags

Magnetic LevitationCell DensityAntigenic PropertiesMinimally InvasiveRhesus FactorFluorescent DyeDPBSHBSSGadoliniumIgG Control BeadsAnti-RhD Coated BeadsCapillary TubeCell SeparationCell DetectionAnemiaSepsis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/62550?article_type=v

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
check_url/fr/62550?article_type=v

Quantifying Mixing using Magnetic Resonance Imaging
check_url/fr/62550?article_type=v

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
check_url/fr/62550?article_type=v

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
check_url/fr/62550?article_type=v

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
check_url/fr/62550?article_type=v

Cellular Redox Profiling Using High-content Microscopy
check_url/fr/62550?article_type=v

Fish Sperm Assessment Using Software and Cooling Devices
check_url/fr/62550?article_type=v

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
check_url/fr/62550?article_type=v

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
check_url/fr/62550?article_type=v

Quantification of Interbacterial Competition using Single-Cell Fluorescence Imaging

Read Article