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Chapitres
- 00:04Introduction
- 00:42Virus Acquisition, Collection of Viruliferous Insects, and Reagent Preparation
- 01:24Dissection of Adults and Excision of Guts
- 02:38Dissection of Nymphs and Excision of Guts
- 03:47Labeling Protocols for SRBSDV Virions and an Insect Protein
- 05:33Results: Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
- 06:32Conclusion
This protocol for immunofluorescent labeling of both plant virus proteins and vector insect proteins in excised insect guts can be used to study interactions among virus and vector insects, insect protein functions and molecular mechanisms underlying virus transmission.
