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Immunologie et infection

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通过荧光原位杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用

Journal JoVE
Immunologie et infection

This content is Free Access.

Journal JoVE Immunologie et infection

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

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通过荧光原位杂交、凝集素染色和成像可视化肠道微生物群与宿主的相互作用

DOI:

10.3791/62646-v

•

09:31 min

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July 09, 2021

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Katharine M. Ng², Carolina Tropini^2,3

¹School of Biomedical Engineering,University of British Columbia, ²Department of Microbiology and Immunology,University of British Columbia, ³Humans and the Microbiome Program,Canadian Institute for Advanced Research (CIFAR)

Chapitres

00:04Introduction
00:56Tissue Sample Collection and Fixation
02:04Paraffin Infiltration and Embedding
02:59Preparation of Slides for Staining and Host Features
04:31Bacterial Staining with FISH
06:41Results: Analysis of the Localization of Muribaculum intestinale Relative to Mucus in a Gnotobiotic Mouse Model and Some Common Imaging Problems
08:33Conclusion

Summary

Traduction automatique

这种简化的实验方案详细介绍了检测和成像复杂组织样品中细菌的工作流程，从固定组织到用荧光原位杂交染色微生物。

Tags

Gut Microbiota Host-associated Microbes Fluorescence In Situ Hybridization (FISH)Lectin Staining Imaging Host-microbiome Interface Methacarn Fixation Paraffin Embedding Intestinal Sections Visualization

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