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Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy
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Journal JoVE Médecine
Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

Visualizing Membrane Ruffle Formation using Scanning Electron Microscopy

DOI:
10.3791/62658-v

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08:05 min

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May 27, 2021

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, , ,
1Vascular Biology Center,Medical College of Georgia at Augusta University, 2Department of Cellular Biology and Anatomy,Medical College of Georgia at Augusta University, 3Department of Pharmacology and Toxicology,Medical College of Georgia at Augusta University

Chapitres

  • 00:04Introduction
  • 00:47Cell Treatment
  • 01:56Scanning Electron Microscopy Fixation
  • 02:48Critical Point Drying
  • 05:16Imaging and Quantification
  • 05:58Results: Visualization and Quantification of Membrane Ruffle Formation
  • 07:34Conclusion

Summary

Traduction automatique

Traduction automatique

Macropinocytosis is a highly conserved endocytic process initiated by the formation of F-actin-rich sheet-like membrane projections, also known as membrane ruffles. Increased rate of macropinocytotic solute internalization has been implicated in various pathological conditions. This protocol presents a method to quantify membrane ruffle formation in vitro using scanning electron microscopy.

Tags

Membrane RufflesMacropinocytosisScanning Electron MicroscopyCell SurfaceSEM ImagingMembrane ActivitiesMacropinocytic CupsMacrophagesPMAM-CSFCritical Point Drying

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