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Cancer Research

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JoVE Journal Cancer Research
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
 

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Article DOI: 10.3791/62663-v • 13:53 min • August 26th, 2021
August 26th, 2021 •
1,2, 1, 1
1Department of Chemical Pathology, Faculty of Health Sciences, University of Pretoria, 2Tshwane Academic Division, National Health Laboratory Service

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résumé
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Apoptosis can be characterized by flow cytometric analysis of early and late apoptotic biomarkers. The cervical cancer cell line, SiHa, was analyzed for apoptosis biomarkers after treatment with Actinomycin D using a benchtop flow cytometer.

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Keywords: Flow Cytometry Apoptosis Actinomycin D SiHa Cervical Cancer Cells Biomarkers Annexin V Cell Culture Trypsin Phosphatidylserine DNA Binding Dyes
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