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일차 및 불멸화 된 인간 각막 상피 세포에 대한 UV 방사선 및 화학 물질의 독성 결정
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Journal JoVE
Biologie
Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
일차 및 불멸화 된 인간 각막 상피 세포에 대한 UV 방사선 및 화학 물질의 독성 결정
DOI:
10.3791/62675-v
•
09:31 min
•
July 22, 2021
•
Jaclyn M. L. Chang
,
Junghee Seo
,
Maggie Miu Yee Kwan
,
Sarah Oh
,
David J. McCanna
,
Lakshman Subbaraman
,
Lyndon Jones
2
1
Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science
,
University of Waterloo
,
2
Centre for Eye and Vision Research (CEVR)
Chapitres
00:05
Introduction
00:56
Determination of Cell Size Using Confocal Microscopy
01:59
Exposure of Cells to Ultraviolet (UV) Radiation
03:50
Exposure of Cells to Chemical Toxins
05:31
Examination of Cells Exposed to Various Concentrations of BAK
06:53
Results: Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
08:50
Conclusion
Summary
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이 기사에서는 1차 및 불멸화 세포주에서 UV 방사선 및 화학 독소의 독성을 평가하는 데 사용되는 절차를 설명합니다.
Tags
Toxicity
UV Radiation
Chemicals
Primary Human Corneal Epithelial Cells
Immortalized Human Corneal Epithelial Cells
In Vitro Assay
Cell Viability
Annexin Staining
Confocal Microscopy
Cytokine Release
Article
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