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JoVE Journal Neuroscience
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
 

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

Article DOI: 10.3791/62744-v • 07:43 min • November 20th, 2021
November 20th, 2021 •
1, 1
1DBT-National Brain Research Centre

résumé
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A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C. elegans.

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Keywords: Microtubule Dynamics Microtubule Orientation Caenorhabditis Elegans Neurons In Vivo Assessment Cytoskeleton Alpha And Beta Tubulin End-binding Proteins EBP-2 GFP PLM Neurons Make-3 Promoter Mounting Polystyrene Beads Fluorescence Microscopy Spinning Disk Unit
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