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A Permanent Window for Investigating Cancer Metastasis to the Lung
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1Department of Anatomy and Structural Biology,Einstein College of Medicine / Montefiore Medical Center, 2Gruss-Lipper Biophotonics Center,Einstein College of Medicine / Montefiore Medical Center, 3Department of Surgery,Einstein College of Medicine / Montefiore Medical Center, 4Integrated Imaging Program,Einstein College of Medicine / Montefiore Medical Cente, 5Department of Pathology,Einstein College of Medicine / Montefiore Medical Center
Chapitres
- 00:04Introduction
- 00:59Lung Window Surgery
- 04:46Results: Assessment of Tumor Cell Migration
- 06:02Conclusion
Here, we present a protocol for the surgical implantation of a permanently indwelling optical window for the murine thorax, which enables high-resolution, intravital imaging of the lung. The permanence of the window makes it well-suited to the study of dynamic cellular processes in the lung, especially those that are slowly evolving, such as metastatic progression of disseminated tumor cells.
