Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / In Vivo Immunogenicity Screening of Tumor-Derived …
JoVE Journal
Cancer Research

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Cancer Research
In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells
 

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Article DOI: 10.3791/62811-v • 08:02 min • September 23rd, 2021
September 23rd, 2021 •
1,2, 1,2,3,4, 1,2
1Department of Medicine III, School of Medicine, Technical University of Munich, 2Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 3Department of Internal Medicine III, University Hospital Regensburg, 4National Centre for Tumor Diseases WERA

Chapitres

résumé
Automatic Translation

This manuscript describes how to assess in vivo immunogenicity of tumor cell-derived extracellular vesicles (EVs) using flow cytometry. EVs derived from tumors undergoing treatment-induced immunogenic cell death seem particularly relevant in tumor immunosurveillance. This protocol exemplifies the assessment of oxaliplatin-induced immunostimulatory tumor EVs but can be adapted to various settings.

Tags

Keywords: - Tumor-derived Extracellular Vesicles - Flow Cytometry - Splenic T Cells - In Vivo Immunogenicity Screening - B16 Melanoma Cells - Ovalbumin - Oxaliplatin - Ultracentrifugation - EV Isolation - Exosome Isolation Reagent - Immunization
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter