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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

DOI:
10.3791/62820-v

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06:28 min

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September 24, 2021

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, , , , , ,
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences,Niigata University, 5Faculty of Medicine,University of Tsukuba

Summary

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The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

Tags

Keywords: Primary Oral KeratinocytesMouse PalateStem Cell-like StateLong-term CultureEpithelial LayerTrypsin DigestionLamina PropriaCell IsolationCulture Technique

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