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Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Isolering af menneskelige neutrofiler fra fuldblod og buffy frakker

DOI:
10.3791/62837-v

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08:12 min

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September 17, 2021

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, , ,
1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Chapitres

  • 00:04Introduction
  • 00:45Gradient Layering
  • 02:40Erythrocyte Sedimentation
  • 03:45Residual RBC Lysis
  • 05:06Quality Determination of Isolated Neutrophil
  • 05:44Results: Neutrophil Yield and Quality Estimation
  • 07:34Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol beskriver en metode til isolering af neutrofiler fra fuldblod, buffy frakker, eller leukaferse membraner, opnå godt udbytte, høj renhed, og minimal celle aktivering. Vi bruger gradient rensning, rød blodlegemer (RBC) sedimentering, og RBC lysis at opnå en høj kvalitet / renhed neutrophil forberedelse.

Tags

NeutrophilsDensity GradientSedimentationLysisBuffy CoatPBMCErythrocyteCell BiologyCell IsolationCell Preparation

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