Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Ramya Ganesan; Martin Kaltenpoth; Laura V. Fl&#243;rez

doi:10.3791/62843

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Transposoninnsetting Sekvensering som et verktøy for å belyse bakteriekoloniseringsfaktorer i en Burkholderia gladioli Symbiont av Lagria villosa Beetles

Journal JoVE
Biologie

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Journal JoVE Biologie

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Transposoninnsetting Sekvensering som et verktøy for å belyse bakteriekoloniseringsfaktorer i en Burkholderia gladioli Symbiont av Lagria villosa Beetles

DOI:

10.3791/62843-v

•

09:55 min

•

August 12, 2021

•

Ramya Ganesan, Martin Kaltenpoth², Laura V. Flórez³

¹Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution,Johannes Gutenberg University, ²Department of Insect Symbiosis,Max Planck Institute for Chemical Ecology, ³Department of Plant and Environmental Sciences, Section for Organismal Biology,University of Copenhagen

Chapitres

00:04Introduction
00:30Conjugation to Generate the Transposon Mutant Library
03:32Mutant Pool Infection on Beetle Eggs
04:43Sequencing Library Preparation
08:34Results: Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors
09:11Conclusion

Summary

Traduction automatique

Dette er en tilpasset metode for å identifisere kandidatens insektkoloniseringsfaktorer i en Burkholderia gunstig symbiont. Billeverten er infisert med et tilfeldig mutantbibliotek generert via transposonmutagenese, og bibliotekets kompleksitet etter kolonisering sammenlignes med en kontroll dyrket in vitro.

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